Process of preparing extracts of the suprarenal glands.



JM 0. V, 185.

UNITED STATES Ia-ucuutzu. 0 MILE 4O, LUUU.

PATENT OFFIC JOKICHI TAKAMINE, OF NEW YORK, .N. Y.

SPECIFICATION forming part of Letters Patent No. 730,198, dated une 2,1903. Original application filed November 5, 1900,,Serial No. 35.546.Divided and this application filed January 8,1001. Serial No. 42,550.(No specimens.)

To all whom, it may concern: v

Be it known that I, J QKIOHI TAKAMINE, a

" subject of the Emperor of Japan, residing in the city of New York,county and State of New York, have invented certain new and usefulImprovements in Processes of Preparing an Extract of the suprarenalGlands; andI do hereby declare the following to be a full, clear,

and exact description of the invention, such as will enable othersskilled in the art to which itappertains to make and use the same.

This invention relates to an improved pro- I cess of preparing anextract of the suprarenal of the process therein described, but. muchpurer than the ordinary aqueous extract, and is suitable for somepurposes.

In carrying out myinvention the clean suprarenal capsules fromanimals--su ch as cat tle, sheep, &c.-are disintegrated by suitablemeans. I then make a fluid extract of the capsules or glands by the useof any suitable extractive solvent for the active principlesuch, forinstance, as water. This I accomplish by mixing with the disintegratedcapsules or glands about the same weight of water and steeping at atemperature of about to centigrade for the period of about five to tenhours in a suitable vessel, preferably avoiding contact with atmosphericair, which can be to a great extent accomplished by a film of fatfloating on top of the liquid or may be done by passing a slow currentof hydrogen or carbonic-acid gas into thetop part of the vessel. Theobject of this is to prevent the oxidation of the extract, At the latterpart of the steeping the temperature of the mixture may be raised from.to centigrade. The massis nowstrained, and

the residue is pressed to squeeze out as much liquid'as possible. Theresidue thus pressed out is steeped again with the least amount of waterto cover the mass for several hours at the same temperature as above.The two extracts thus obtained are mixed, and.-the,mix-.- to re iscooled rapidly and the solidified fat removed. The liquid is nowevaporated at alow temperature, preferablyin a vacuum-pan, ld-a mitting,ifvnecessary, a small quantity of hydrogen or carbonic acid to replaceair andt'to:

prevent oxidation. until it becomes one-fifth to one-seventh oftheoriginal volume. To this concentrated solution two to three times itsownvolume of al-.

The liquid is evaporated This addition of alcohol will precipi-,

then filtered and the filtrate distilledninder i vacuum to separate thealcohol, which is condensed andrecovered in' asuitable manner.

This concentration is carried as far as may be desirable, and theproduct may be sold in Q any desired degree of concentration, or bycarrying the concentration sufliciently far the:

product may be reduced to a solidstate and maybe sold in that form.

- 75 Having thus described myinvention, the

following is what I claim asrnew therein and desire to secure by LettersPatent:

1. The process of preparing an extracto suprarenal glands, whichconsists in making a fluid extract of the glands, addingto said,

extract a precipitant for nonactive a-lbu minoid and mineralmatters, andremoving the,

precipitate thus formed.

2. The'process ofpreparingan extract of;

the suprarenal glands, which consists inmakving an aqueous extract oftheglands,adding to said extract a precipitant for'non-active albuminoidand mineral matters and-removing the precipitate so formed.

3. The process of preparingan extra-ct of centrating itto suitablestrength, adding alcohol to precipitate non-activealbuminoid thesuprarenal glands, which consists in;making an aqueous extract of theglands, con-- and mineral matters, and ;removingt he-pre cipitate,substantially asset forth.

4. The process of preparing an extract of the suprarenalglands,wl1ich-consists in make ing an aqueous extract of the glands,concentrating it to suitable strength, adding alcohol to precipitatenon-active albuminoid and mineral matters, filtering, and concentratingthe filtrate by evaporation, substantially as set forth.

5. The process of preparing an extract of the snprarenal glands, whichconsists in disintegrating the glands, steeping the disintegrated glandsin Water While preserving the same from oxidation, concentrating theextract so produced, adding alcohol to the concentrated extract toprecipitate non-active 10 bodies, removing the precipitate so formed andconcentrating the residue.

JOKICHI TAKAMINE. Witnesses:

ARTHUR P. KNIGHT, J. GREEN.

